Comparative Analysis of Ginsenosides in Ginseng Radix et Rhizoma and Ginseng Radix et Rhizoma Rubra by UFLC-QTRAP-MS/MS / 中国药学杂志

ABSTRACT

OBJECTIVE To develop a comprehensive analytical method based on UFLC-QTRAP-MS/MS for simultaneous determination of protopanaxadiol [ginsenoside Rb1, Rc, Rb2, Rd, F2, 20(S)-Rg3, 20(R)-Rg3, CK], protopanaxatriol [ginsenoside Re, Rg1, Rf, 20(S)-Rg2, 20(S)-Rh1, 20(R)-Rg2, 20(R)-Rh1, F1] and oleanolic(ginsenoside Ro) in Ginseng Radix et Rhizoma and Ginseng Radix et Rhizoma Rubra. METHODS Under the optimized chromatographic conditions, good separation for seventeen target compounds was obtained on a SynergiTM Hydro-RP 100 column(2.1 mm×100 mm, 2.5 μm) at 40 ℃ with 0.1% aqueous formic acid (A)/acetonitrile (B) as the mobile phase by gradient elution at a flow rate of 0.4 mL·min-1. The target compounds were analyzed under multiple reaction monitoring (MRM) mode with an ESI source operated in negative ion mode, and principal component analysis (PCA) and hierarchical cluster analysis (HCA)were used for data processing. RESULTS The calibration curves of the 17 components had good linearity (r>0.999 0). The precision, repeatability and stability were all satisfying.The average recoveries of standard addition for the compounds were between 96.69% and 102.01%,and the relative standard deviations were less than 5%. The results of PCA and HCA showed that Ginseng Radix et Rhizoma and Ginseng Radix et Rhizoma Rubra were clearly distinguished.The main compositions with significant difference were ginsenoside 20(S)-Rg3, 20(R)-Rg3, 20(S)-Rh1, 20(R)-Rh1, and 20(R)-Rg2. CONCLUSION The established method could provide a new technique for the comprehensive evaluation and quality control of Ginseng Radix et Rhizoma and Ginseng Radix et Rhizoma Rubra, at the same time, it would pave the way for discovering the material basis contributing to the different properties and efficacies of the two medicinal materials.