Determination of Related Substances of Azithromycin for Injection by Gradient RP-HPLC / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 1300-1308, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-858258
ABSTRACT
OBJECTIVE:
To establish a gradient elution method for determination of related substances of azithromycin for injection.METHODS:
Gradient elution was used for the analysis. A C18 (CAPCELL PAK MGⅡ, 4.6 mm×250 mm,5 μm)column was used. The mobile phase A consisted of 0.05 mol·L-1 K2HPO4 solution (pH 8.2, adjusted with 20% phosphoric acid)-acetonitrile (45∶55), and the mobile phase B was methanol. The flow rate was 1.2 mL·min-1, and the detection wavelength was set at 210 nm. The column temperature was maintained at 30 ℃. The gradient elution program was as follows 0-35 min, A75%→95%; 35-64 min, A75%; 64-65 min, A95%→75%; 65-71 min, A75%.RESULTS:
Azithromycin, near peaks and known impurities were well separated from each other. All acid radicals of azithromycin for injection did not influence the determination of related substances of azithromycin.CONCLUSION:
This method is more specific than the exising method for determination of related substances of azithromycin, which can more effectively control the quality of the drug.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Pharmaceutical Journal
Año:
2018
Tipo del documento:
Artículo
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