Molecular Identification of Belamcandae Rhizoma, Iridis Tectori Rhizoma and Their Adulterants Based on ISSR and SCoT Analysis / 中国药学杂志

ABSTRACT

OBJECTIVE: To identify Belamcandae Rhizoma, Iridis Tectori Rhizoma and their adulterants by ISSR and SCoT markers. METHODS: The genome of Belamcandae Rhizoma, Iridis Tectori Rhizoma and its adulterants were amplified by the optimized ISSR and SCoT PCR conditions and screened primers. Genome polymorphism analysis and the dendrogram construction were calculated by the POPGENE 1.32 and the NTSYS-pc version 2.10 respectively. RESULTS: The 130 and 143 bands were amplified by the screened ISSR and SCoT primers respectively. The percentage of polymorphic bands were 96.2% and 97.9% respectively. Shannon diversity index(I) were 0.540 2 and 0.500 3, Nei's gene diversity index (H) were 0.363 0 and 0.327 3 respectively. The genetic distance calculated based on SCoT marker was higher than that of ISSR marker, which suggested that Elamcandae Rhizoma, Iridis Tectori Rhizoma and their adulterants have great genetic diversity. Cluster analysis based on SCoT genetic similarity indicated that Belamcandae Rhizoma, Iridis Tectori Rhizoma formed independent group with far relationship among their adulterants. CONCLUSION: Belamcandae Rhizoma, Iridis Tectori Rhizoma and their adulterants could be distinguished stablely, fastly and clearly by SCoT marker. The ISSR marker could only be used for identification of Belamcandae Rhizoma and its adulterants. The SCoT marker is better suitable for genetic diversity research on the low taxonomic category in genus Iris.