The Protective Effect of Zhizichi Decoction on the Apoptosis of PC 12 Cells Induced by Glutamic Acid / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the protective effect of Zhizichi Decoction extract against glutamic acid-induced neurotoxicity in PC 12 cells. METHODS: PC 12 cells were treated with glutamic acid to establish the cell model. Cell viability and membrane permeability were examined by MTT assay and LDH kit. The apoptosis rates were measured by flow cytometry using fluorescein labeling FITC-Annexin V/PI. The production of ROS was determined by flow cytometry. The levels of GSH and SOD in the cells were determined by kit respectively. RESULTS: Zhizichi Decoction extract significantly decreased the contents of LDH, enhanced the activities of SOD and GSH induced by glutamate. In addition, Zhizichi Decoction extract treatment decreased the intracellular ROS generation. CONCLUSION: Zhizichi Decoction extract can significantly ameliorate glutamic acid induced PC 12 cells injury. The mechanism may be partly through anti-oxidative stress.