Effect of Dracocephalum heterophyllum Benth Flavonoid on Rat Myocardial Cell Hypertrophy Induced by Angiotensin Ⅱ / 中国药学杂志

ABSTRACT

OBJECTIVE: To observe the effect of Dracocephalum heterophyllum flavonoids(DHBF) of Uygur Medicine on cardiomyocyte hypertrophy, which were induced by angiotensin Ⅱ(AngⅡ), and it could provide a basis for further study to the mechanism. METHODS: SD rats, 0 - 3 d of age, neonatal rat myocardial cells cultured in vitro, the experiment was divided into control group, AngⅡ(1 μmol•L-1) group, different concentrations of DHBF(10, 25, 50 μmol•L-1) + AngⅡ(1 μmol•L-1) groups, cardiomyocyte hypertrophy were induced by AngⅡ 1 μmol•L-1 and was intervened using DHBF respectively, CCK-8 method was used to observe the activity of myocardial cells, RT-PCR technique was used to detect the expression of m RNA of cardiac hypertrophy gene atrial natriuretic peptide(ANP) and brain natriuretic peptide(BNP), the internal factor was glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Confocal laser scanning was used to detect the surface area of myocardial cell was [Ca2+]i; the activity of Ca2+-ATP was measured by the enzymatic reaction of fragmentation cell; the concentration of NO and the activity of NOS were determined by colorimetry. RESULTS: Compared with the control group, the activity of myocardial cell was(85% ± 5%) in the AngⅡgroup, which was increased significantly after it was dealed with DHBF and AngⅡ(P < 0.05); RT-PCR results showed the expression of mRNA of ANP and BNP were increased by using AngⅡ, which were lower by using DHBF and AngⅡ. The surface area of myocardial cell were increased by using AngⅡ, which could be reversed by using DHBF and AngⅡ. Confocal laser scanning showed the concentration of [Ca2+]i was increased by using AngⅡ, but which was lower significantly by using DHBF and AngⅡ(P < 0.05). The enzymatic reaction of fragmentation cell results showed the activity of Ca2+-ATP was decreased by using AngⅡ, but which was increased significantly by using DHBF and AngⅡ(P < 0.05). Colorimetry results showed the concentration of NO and the activity of NOS were decreased by using AngⅡ, but which was increased significantly by using DHBF and AngⅡ(P < 0.05). CONCLUSION: DHBF can improve the activity of hypertrophy cardiomyocytes which were induced by angⅡ, downregulate expression of mRNA of ANP and BNP, reduce surface area of cardiomyocytes induced by AngⅡ, the mechanism of action may be related to promoting the release of NO, regulating the concentration of[Ca2+]i and the activity of Ca2+-ATP.