Determination of Astragaloside in Four Kinds of Chinese Patent Medicines by ASE-CAD Combined with Column Post Compensation Liquid Chromatography / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 456-459, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-858396
ABSTRACT
OBJECTIVE:
To establish a method for determination of astragaloside in 4 Chinese patent medicines (BuZhong YiQi Pills, TongQiao BiYan Granules, XingNao ZaiZao Capsules, YuPing Feng Granules) by accelerate solvent extraction (ASE) combined with column post compensation liquid chromatography and charged aerosol detector (CAD) detection, and compare the result with that of the pharmacopoeia method.METHODS:
The optimal extraction conditions were determined by the ASE test water saturated n-butanol was used as solvent, ASE extraction temperature was 100℃, extraction time was 7 min and cycle time was 3. For the HPLC analysis, Thermo AQ-C18 column (2.1 mm × 100 mm, 3 μm) was employed, the mobile phase for the analysis pump was composed of acetonitrile-water (1585), and the flow rate was 0.3 mL•min-1. Gradient program was as follows 0 min, 15% A; 0 - 4 min, 15% - 60%A; 4 - 5 min 60% A. The mobile phase for the compensating pump was acetonitrile, and the flow rate was 0.3 mL•min-1.RESULTS:
The linear range of the calibration curve of astragaloside was 26.62 - 665.5 μg•mL-1. The RSD of sample analysis was 1.0% - 2.1%. The average recoveries were 98.80% - 100.80%. The method is in good agreement with the Pharmacopoeia method.CONCLUSION:
The method is rapid, accurate and reproducible. It can be used for the determination of astragaloside in 4 kinds of Chinese patent medicines.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Pharmaceutical Journal
Año:
2018
Tipo del documento:
Artículo
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