Protective Effect of Mullein Glycoside Polysaccharide Extracting from Cistanche on PC12 Nerve-Cell Damage Model Induced by D-galactose / 中国药学杂志

ABSTRACT

OBJECTIVE: To explore the protective effect of mullein glycoside polysaccharide of Cistanche deserticola Ma on PC12 nerve-cell model induced by D-galactose. METHODS: The cell survival rate was determined by MTT assays, which provided the basis for selecting mullein glycoside polysaccharide dose and estimated the dose and action time of D-galactose for inducing PC12 nerve cell damage model. After mullein glycoside polysaccharide incubation of PC12 cells, western blotting was used to detect the levels of CREB and p-CREB protein expression. ELISA Kit was used to detect the levels of cyclic adenosine monophosphate(cAMP), cAMP dependent protein kinase(PKA) and brain derived neurotrophic factor(BDNF). The content of MDA, activities of SOD and LDH were measured by their respective kits. RESULTS: (1)After the exposure of the PC12 cells to 16 g·L-1 D-galactose for 40 h, the cell survival rate was (46.67±6.59)%, which has a significant difference compared with the control group(P<0.05), indicating that successful cell aging model was established. (2)Compared with those in model group, mullein glycoside polysaccharide could significantly increase p-CREB expression in dose-dependent manner(r=0.989, P<0.01), content of PKA, cAMP, BDNF and SOD and decrease the levels of MDA and LDH(rMDA=0.875, P<0.05);(rLDH=0.834, P<0.05). However, blockers H-89 could significantly decrease p-CREB expression, PKA, cAMP, SOD and BDNF content(P<0.05), and increase the levels of MDA and LHD(P<0.05). CONCLUSION: The mullein glycoside polysaccharide of Cistanche deserticola Ma has obvious protective effect on PC12 nerve-cell damage model induced by D-galactose and its mechanism relates to the upregulation of cAMP/PKA/ CREB signaling pathways.