Mechanism Study of Supernatants of Alcohol Extracting-Water Precipitation of Polygonatum odoratum on Hyperthyroidism-yin Deficiency Rats Based on GC-MS Metabonomics / 中国药学杂志

ABSTRACT

OBJECTIVE: To evaluate the effect of supernatants of alcohol extracting-water precipitation of Polygonatum odoratum(POS) on hyperthyroidism-yin deficiency rats. METHODS: SD rats were randomly divided into control, model and POS groups(400 mg·kg-1). The hyperthyroidism-yin deficiency rats were treated by intraperitoneal injection of T3(500 μg·kg-1) for a week. The content of T3, T4 in serum and cAMP, cGMP in plasma was determined. A GC-MS based metabolic profiling method was employed to analyze the distinctive metabolic patterns of serum in control, model and POS. Multivariate statistical analysis was applied for multivariate analysis to find potential biomarkers of hyperthyroidism-yin deficiency by SIMCA-P 12.0 software. RESULTS: Compared with the model group, POS group can significantly reduce the content of T3, T4, cAMP, the ratio of cAMP/cGMP and increase the content of cGMP, indicating it has certain Yin-nourishing action. The PCA and PLS-DA score plots showed metabolic profiling of control, model and POS had significant differences, but which could be regulated nearly to normal state after POS interference. Four potential biomarkers were identified by PLS-DA and variable importance for project values(VIP) based on the metabolomics. They were 3-hydroxybutyric acid, galactose, palmitic acid and cholesterol. CONCLUSION: POS may play a role in treating hyperthyroidism-yin deficiency rats, by adjusting energy metabolism, glucose metabolism and lipid metabolism. The changes of metabolic pathways revealed by this study may provide some basis for the diagnosis and treatment of hyperthyroidism Yin-deficiency.