Construction of Interferon-Resistant HBV Cell Model / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an interferon-resistant hepatitis B virus cell model and provide experimental basis for further investigating the mechanism of HBV resistance to interferon. METHODS: HepG2.2.15 was continuously grown in the presence of 10, 30, 50 and 70 u·mL-1 of interferon α-2b for up to 48 weeks. The HepG2.2.15/IFNα-2b cell model was constructed after 48 weeks of induction. The cells were treated with the best-effect concentration. Then, the levels of HBsAg, HBeAg, and HBV DNA in the supernatant of cell culture medium before and after the treatment were compared. RESULTS: After stimulation with low concentrations of IFNα-2b for 12 weeks, the 50 u·mL-1 group showed significant resistance to the best-effect concentration of IFNα-2b. Compared with the levels before stimulation, the inhibition rate on HBsAg, HBeAg, and HBV DNA decreased by 25.48%, 8.40%, and 15.43%, respectively, suggesting that 50 u·mL-1 was the best-stimulation concentration. After stimulated with 50 u·mL-1 IFNα-2b for 12-48 weeks, the results showed that the inhibition rate on HBsAg, HBeAg, and HBV DNA after 36 weeks was the most significant. CONCLUSION: Continuous induction with 50 u·mL-1IFNα-2b for 36 weeks could most easily induce drug resistance in HepG2.2.15 cells.