Intratracheal Atomization of Lipopolysaccharides Induced Lung Inflammatory Injury in Rats Model / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish dose-related lung inflammatory injury in rats model with intratracheal atomization of lipopo-lysaccharide (lipopolysaccharides, LPS). METHODS: Four groups of 4 rats were subjected to solvent or a single dose of LPS by in- tratracheal route using a IA-1B-2 inches-microsprayer. The male rats received 200 μL solvent (control), LPS solutions (15, 5, 0.5 mg·kg-1). All rats were sacrificed 24 h after dose administration, biochemical analysis and cell counts on bronchoalveolar lavage fluid (BALF) were performed on each rat. Lung, trachea and kidney were examined histologically. Serum chemistry profiles of creatinine, ALB, Na, K, Cl-were detected. RESULTS: Cell counts in BALF showed LPS groups had different degrees of inflammatory reaction. The alkaline phosphatase and total protein concentration were higher in LPS high dose group compared with other groups. In addition, the concentration of TNF-α increased consistently with LPS dose and has statistical significance compared with the control group. Histopathology findings demonstrated that LPS produced an accumulation of foamy macrophages in the lungs and high degree of inflammation. CONCLUSION: The results recommends intratracheally atomizing doses of LPS in rats model produced ranks of lung inflammatory injury.