Apoptosis of human gastric cancer cell line BGC-823 induced by liposome of total glucosides from Paeonia / 中国药学杂志

ABSTRACT

OBJECTIVE: To study the apoptosis of human gastric cancer cell line BGC-823 induced by liposome of total glucosides from paeonia(TGP liposome). METHODS: The inhibition of cell proliferation was determined by Cell Counting Kit-8 assay. The morphological changes of the apoptosis cells were observed by HE staining, Hoechst 33258 fluorescent staining, transmission electron microscope. Changes of apoptosis related factor Bcl-2 and Bax protein expression were detected by Western blot. RESULTS: TGP liposome significantly inhibited proliferation of BGC-823 cells in a dose-dependent manner(P < 0.05). GC-823 cells induced by TGP liposome for 48 h was observed on morphological changes such as karyopyknosis and karyorrhexis and apoptotic bodies. Bcl-2 expression was reduced, but Bax expression was increased, so the ratio of Bcl-2/Bax decreased. CONCLUSION: TGP liposome can induce the apoptosis of BGC-823 cells and inhibit its proliferation, its mechanism may be related to down-regulating protein expression of Bcl-2, up-regulating protein expression of Bax, reducing Bcl-2/Bax value.