Protective effect of aidi injection on nephrotoxicity induced by cisplatin in rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the protective effects of aidi injection on cisplatin (CDDP)-induced acute nephrotoxicity in rats. METHODS: Forty male Sprague-Dawley rats were randomly divided into 5 groups, including the control group, CDDP group, the low, medium and high dose Aidi injection group (5, 10, 15 mL·kg-1). Except the rats in control group, the rats of other groups were injected with CDDP on the third day. Aidi injection groups were administered to the rats once daily for 7 consecutive days. The control and CDDP groups were given corresponding regime of saline. On the eighth day, determine the content of serum creatinine (Scr) and blood urea nitrogen (BUN) in the rat, detect the level of superoxide dismutase (SOD), glutathione reductase (GSH-Px) and malondialdehyde (MDA) in the kidney tissue; observe HE staining slices of rats and find the renal pathological changes. RESULTS: Compared with control group, the levels of Scr, BUN and MDA content were increased significantly in CDDP group, whereas the contents of SOD and GSH-Px were decreased significantly. There were serious, renal tubular epithelial cell apoptosis and inflammation in the kidney tissue. Kidney histopathology indicated that Aidi injection alleviated CDDP-induced tissue damage. The levels of Scr, BUN were decreased significantly in the low dose Aidi injection group, whereas the activity of SOD and GSH-Px were increased significantly. The medium dose of Aidi injection could increase the level of SOD and GSH-Px. The high dose of Aidi injection could enhance the activity of GSH-Px, without statistically significant effect on other indicators. CONCLUSION: The results indicate that the low and middle dose of Aidi injection might protect the renal tissue from nephrotoxicity induced by cisplatin through anti-oxidation.