Assay and fingerprint chromatogram of scutellaria barbata formula granules by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the HPLC methods for determining the contents of the active ingredients and fingerprint chromatogram of Scutellaria barbata formula granules. METHOD: The HPLC analysis was carried out on a Wondasil C18 column (4.6 mm×250 mm, 5μm) with mobile phase consisting of methanol-acetonitrile-0.1% phosphoric acid (12.51572.5) at the flow rate of 1.0 mL·min-1. The column temperature was maintained at 30t. The detection wavelength was set at 335 nm to determine the contents of scutellarin and scutellarein and 320 nm to establish the fingerprint chromatogram of Scutellaria barbata formula granules, medical materials, and aqueous decoction. RESULTS: The linear ranges of scutellarin and scutellarein were 0.074 0-0.518 0 μg and 0.051 6-0.3612 μg, respectively. The average recoveries were 103.18% and 99.99%, respectively. Nine peaks in the fingerprint chromatogram of formula granules could be tracked in the aqueous decoction, and eight peaks in the fingerprint chromatogram could be tracked in the medical materials. CONCLUSION: The methods can provide more information for the quality control of Scutellaria barbata formula granules.