Changes of secondary metabolites and differential proteomics in mulberry leaves under UV-B induction / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the influence of ultraviolet B (UV-B) induction on the content of two kinds of secondary metabolites in mulberry leaves and differential proteomics under UV-B induction. METHODS: The HPLC chromatograms of secondary metabolites in leaves before and after UV-B induction were established and quantitative analysis of two kinds of secondary metabolites was performed. The subcellular structure of mulberry leaves under UV-B induction was observed using transmission electron microscopy. Proteomic analysis of mulberry leaves was performed using two-dimensonal electrophoresis (2-DE). RESULTS: The subcellular structure of mulberry leaves was destroyed under UV-B induction. The contents of moracin N and chalcomoracin in mulberry leaves were increased with the dark incubation time and they maximized at 36 and 40 h, respectively. The result of proteomics showed that the abundance of chalcone synthase (CHS) which is the key enzyme of flavonoid biosynthesis pathway was increased significantly. CONCLUSION: The secondary metabolism especially for flavonoids biosynthesis in mulberry leaves is changed under UV-B induction and dark incubation, which result in the increased moracin N and chalcomarcin.