Analysis of gene expression of Pesudostellariae Radix from different habitats based on cDNA-AFLP analysis / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the gene differential expression in Pesudostellariae Radix from different habitats, isolate and evaluate associated differential genes. METHODS: cDNA-AFLP technique was applied to analyze the differential expression genes of Pesudostellariae Radix from four different habitats. RESULTS: Six primer pairs were selected and amplified. Thirty-four differentially expressed trivially distributed file system (TDFs) were obtained from Pesudostellariae Radix samples from different habitats. Then these TDFs were cloned and sequenced, and the nucleotide sequences of 21 TDFs were obtained. By BLASTX analysis, 15 of them were found to have homologous sequences in the databases, among which seven TDFs had known function. These proteins were mainly involved in the growth and development of plants and played a role in the metabolisms of defending diseases and insect pests, and improving the ability of plants to resist abiotic stress. CONCLUSION: This research evaluates differential expression genes in Pesudostellariae Radix from different habitats, providing the basic information for revealing the molecular mechanism of the property formation of Pesudostellariae Radix.