Rapid determination of four categories of pesticide residues in toddaliae asiaticae (L.) lam. by carb/NH2-SPE-GC-MS / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a Carb/NH2-SPE-GC-MS method for rapid monitoring of 40 kinds of pesticides of four categories in Toddaliae asiaticae (L.) Lam. METHODS: The samples were pretreated with Carb/NH2 solid phase extraction (SPE) subpost, and a GC-MS method was used for the determination of the trace pesticide residues. RESULTS: In comparison with the pesticide residue pretreatment methods included in the Chinese Pharmacopoeia, the Carb/NH2-SPE-GC-MS method showed no significant differences (P>0.05) and was simpler and more feasible in handling a variety of pesticide residues in Toddaliae asiaticae (L.) Lam. Good linearity was obtained for the calibration curves for the 40 kinds of pesticides in the concentration range of 10 to 200 ng·mL-1 (r>0.998 2), the limits of detections (LODs) were around 2.5 ng·mL-1, and the precisions, stabilities and recoveries basically met the methodological requirements. CONCLUSION: Carb/NH2-SPE-GC-MS method established in this paper is simple, rapid, highly sensitive, and accurate for the qualitative and quantitative analysis of multi-component pesticide residues in Toddaliae asiaticae (L.) Lam.