Antibacterial metabolites of marine fungus penicillium sp. F00120 from the deep sea sediments in the South China Sea / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the antibacterial agents in the marine fungus Penicillium sp. F00120 from the deep sea sediments in the South China Sea. METHODS: The compounds were purified by a combination of chromatographic methods, and their structures were identified by MS, 1H-NMR, and 13C-NMR spectroscopic analysis. The antimicrobial activities of the isolates were evaluated by the agar plate diffusion method. RESULTS: Eight compounds were isolated from the culture and were characterized as ergosta-4, 6, 8(14), 22-tetraen -3-one(1), 25-hydroxyergosta4, 6, 8(14), 22-tetraen-3-one(2), ergosta-7, 22-dien-3β, 5α, 6β-triol (3), ergosta-5, 7, 22-trien-3β-ol(4), 5α, 8α-epidioxyergosta-6, 22-dien-3β-ol(5), 5α, 6α-epoxyergost-8(14), 22-dien-3β, 7α-diol(6), cholesterol(7), and 4-hydroxyacetophenone(8), respectively. CONCLUSION: Compounds 1-3 and 6-8 are isolated from this fungus for the first time. Compounds 3, 4, and 8 shows moderate antibacterial activities against P. aeruginosa, S. aureus, and E. coll.