Authentication of raw material for edible and medicinal cinnamon based on plastid Intergenic Region ps-bA-trnH / 中国药学杂志

ABSTRACT

OBJECTIVE: To introduce DNA barcoding based on plastid intergenic region psbA-trnH to authenticate medicinal and edible materials Cinnamon cassia to promote the standard practice of medication and foods. METHODS: The genomic DNA of eight Cinnamomum species were extracted successfully. The Kimura 2-parameter (K2P) distances were calculated and phylogenetic tree was constructed using software MEGA 5.0. RESULTS: The sequence length of the psbA-trnH of Cinnamomum cassia was 368 bp. The ps-bA-trnH intra-specific genetic distance of C. cassia was highly conservative, which was shorter than the interspecific ones. The NJ tree indicated that Cinnamomi Cortex could be distinguished from its adulterants obviously. CONCLUSION: The psbA-trnH intergenic spacer can be used to distinguish Cinnamomi Cortex from its adulterants accurately.