Determination of Fla-CN in plasma by LC-MS/MS and its application in the pharmacokinetic study in rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an LC-MS/MS method for determination of Fla-CN in plasma. METHODS: Tiliroside from Potentilla chinensis Ser. was used as the internal standard. After protein precipitation by acetonitrile, samples were separated on Agilent XDB-C18 column by using acetonitrile-water (6535) as mobile phase. The quantitation was performed on an LC-MS/MS with negative electrospray ionization in multiple reaction monitoring (MRM) mode. Fla-CN was detected at m/z 454.0→-255.0, tiliroside (IS) at m/z 593.2→285.1. RESULTS: Linear calibration curves were obtained in the concentration range of 0.5-1000 ng·mL-1 for Fla-CN. The inter-day and intra-day precision values (RSD) were below 4.6%, and the accuracy (relative error) was within ±4.1%. The method was used to determine the plasma concentration of Fla-CN after oral administration in SD rats in a predinical pharmacokinetic study. CONCLUSION: The LC-MS/MS method for determination of Fla-CN in plasma is sensitive, specific and suitable for the pharmacokinetic study of Fla-CN in rats.