Protective effects of aucubin on keratinocyte damaged by Ultraviolet B / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the protection of aucubin on keratinocyte damaged by Ultraviolet B and explore its possible mechanism. METHODS: The photo damage model of keratinocyte was established by irradiating of UVB (64 mJ · cm-1). The different concentration aucubin were used for damaged cell. The cell viability was detected by MTT, the SOD activity, GSH-Px activity, CAT activity, MDA content were assayed by kit respectively. The mRNA levels of P38, TNF-α and IL-6 were determined by RT-PCR. The secretion levels of TNF-α and IL-6 of cultured keratinocyte were detected by ELISA. RESULTS: After the UVB irradiation, the cell viability, the activities of SOD, GSH-Px and CAT were decreased, the content of MDA, TNF-α and IL-6, the mRNA levels of P38, TNF-α and IL-6 were increased (P < 0.01). The above changes were inhibited by 1 × 10-1 and 1 × 10-1 mol · L-1 aucubin (P < 0.05 or P < 0.01). CONCLUSION: The aucubin could inhibit the injury by UVB for keratinocyte. And its possible mechanism may have relationship with the inhibition of oxidative damage, regulatory P38 signal pathway, adjusting the expression of TNF-α and IL-6.