Spectrum efficiency capture technology screening anti-inflammatory and anti-asthmatic components of Shedanchenpi oral liquid / 中国药学杂志

ABSTRACT

OBJECTIVE: To develop a simple and dual-target method based on ultra-performance liquid chromatography/quadru-pole time-of-flight mass spectrometry combined with dual-bioactive (NF-κB and β2-adrenergic receptor) luciferase reporter assay system for rapid determination of various bioactive compounds of the traditional Chinese medicine preparation Shedanchenpi oral liquid. METHODS: Potential anti-inflammatory and spasmolytic constituents were screened using NF-κB and β2-adrenergic receptor activity laciferase reporter assay system and simultaneously identified according to the time-of-flight mass spectrometry data. RESULTS: Synephrine had the most obvious inhibition on the activation effect of β2-AR. Cholalic acid, glycocholic acid, taurochenodeoxycholic acid, and taurocholic acid activation displayed the most significant inhibitory effect on the activation effect of NF-κB. CONCLUSION: Dual bioactivity-integrated online spectrum efficiency capture technology is a powerful tool for the improved screening and identification of potential dual-target lead compounds in complex herbal medicines.