Establishment of HPLC speciftic chromatogram of Dendrobium officinale capsules / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the RP-HPLC specific chromatogram of Dendrobium officinale capsules for the quality control. METHODS: An Ultimate C18 column (4.6 mm × 250 mm, 5 μm) maintained at 30°C was used for all analysis. Chromatographic separation was carried out using gradient elution of methanol and 0.2% phosphoric acid aqueous solution at a flow rate of 1.0 mL · min. The detection wavelength was set at 280 nm. The similarity evaluation system for chromatographic fingerprint of TCM (National Pharmacopoeia Committee) was adopted to construct the specific chromatogram. RESULTS: The HPLC specific chromatogram of Dendrobium officinale capsules was constructed with four common specific peaks with naringenin peak as the reference. CONCLUSION: The method shows good precision and repeatability. It can be used for the quality control of Dendrobium officinale capsules and can be taken as a reference for the identification and quality control of other Dendrobium officinale-related health foods.