Simultaneous determination of four effective components in Jishengshenqi tablets by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an HPLC method for simultaneous determination of the contents of loganin, paeoniflorin, cinnamaldehyde, and paeonol in Jishengshenqi tablets traditional Chinese medicines. METHODS: SHISEIDO MG II C18 column(4.6 mm × 250 mm, 5 μm) was used. The mobile phase was acetonitrile and water with gradient elution. The detection wavelengths were set at 238 nm for loganin and paeoniflorin and 280 nm for cinnamaldehyde and paeonol. The column temperature was 30°C and the flow rate was 1.0 mL · min-1. RESULTS: Loganin, paeoniflorin, cinnamaldehyde, and paeonol showed good linear relationships within their respective linear ranges. The average recoveries were 97.6%, 100.5%, 101.9%, and 102.8%, respectively. CONCLUSION: The method is simple, accurate, repeatable, and can be used to control the quality of Jishengshenqi tablets.