HPLC fingerprint of flavonoids in Dendrobium officinale from Zhejiang Province / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish the HPLC fingerprint of flavonoids from Dendrobium officinale and screen important flavonoid components. METHODS: HPLC analysis was carried out on an XB C18 column(4.6 mm × 250 mm, 5 μm) with methanol and water containing 0.4% formic acid as mobile phase using gradient elution program. The detection wavelength was set at 335 nm. Total 11 batches of Dendrobium officinale and 15 batches of different species of Dendrobium were analyzed. The similarity evaluation system for chromatographic fingerprint of Traditional Chinese Medicine(2004AB) was applied to analyze the similarity. And principal component analysis (PCA) was used in data analysis. RESULTS: The HPLC fingerprint of flavonoids in Dendrobium officinale was established. Six peaks in this HPLC fingerprint were selected as common characteristic peaks, five of which were identified by HPLC-DAD-ESI2. Among the five peaks, four were flavone di-C-glycosides whose aglycone was apigenin, and the other one was naringenin. There were significant differences in the fingerprint chromatograms between Dendrobium officinale and different species of Dendrobium. PCA results indicated that apigenin-6,8-di-C-β-D-glucoside, schaftoside and naringenin were the most important components. CONCLUSION: The established method is highly characteristic and can provide reference for improving the quality control of Dendrobium officinale.