In vitro effects of galangin on cell proliferation, cycle progression and apoptosis of a human gastric cancer SGC-7901 cells / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the effect of galangin on the anti-proliferation and apoptosis of gastric cancer SGC-7901 cells, and possible mechanisms. METHODS: Proliferative activity of SGC-7901 cells was measured by MTT assay while that cell cycle progression and apoptosis of galangin-treated SGC-7901 cells were analyzed by flow cytometry, morphologic observation and mitochondrial membrane potential (MMP) analysis. RESULTSS The evaluated results showed that when galangin was added into cell culture in 40-200 μmol · L-1, proliferative inhibition occurred and was observed as a dose- and time-dependent manner. The calculated IC50 values for galangin were 160, 100 and 70 μmol · L-1 when the treatment time was 24, 48 and 72 h, respectively. Flow cytometry analysis revealed that the proportion of the cells in the G2/M phase was significantly enhanced from 4.40(control group) to 18.31 (treatment group) by a treatment time of 24 h. CONCLUSION: The cells in treatment group showed typical apoptotic morphology and a decrease in MMP. At the same time, the percentage of the apoptotic cells significantly increased from 2.6% or 4.3% (control group) to 27.4% or 65.6% (treatment group), when 160 μmol · L-1 galangin treated SGC-7901 cells for 24 or 48 h. These mentioned results indicate that galangin can inhibit the proliferation and induce apoptosis of the SGC-7901 cells by perturbation in cell cycle progression and mitochondrial dysfunction.