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Effects of TREM-1 gene silencing on lipopolysaccharide-induced inflammatory factor secretion from mice macrophage cell lines RAW264.7 / 中国药学杂志
Chinese Pharmaceutical Journal ; (24): 695-699, 2013.
Artículo en Chino | WPRIM | ID: wpr-860394
ABSTRACT

OBJECTIVE:

To investigate the role of TREM-1 in tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) secretion from lipopolysaccharide-induced mice macrophage cell lines RAW264.7.

METHODS:

Designing and synthesizing small interfering RNA (siRNA) with high intangerference ratio, then constructing pLKO1.1-puro-TREM-1 The mice macrophage cell lines RAW264.7 were divided into four groups control group (control); lipopolysaccharide group (LPS); empty plasmid group (pLKO1.1) - just transfected with pLKO1.1; interference group (siRNA) - transfected with pLKO1.1-puro-TREM1.24 h after stimulation with LPS, real-time PCR was used to detect the mRNA levels of TREM-1, TNF-α and IL-1β respectively. The concentrations of TNF-α and IL-1β were assayed by ELISA.

RESULTS:

In siRNA group, the mRNA levels of TREM-1, TNF-α and IL-1β were decreased significantly (P<0.01) moreover, the concentrations of TNF-α and IL-1β were lower than other groups significantly (P<0.01).

CONCLUSION:

Small interfering RNA may reduce TNF-α, IL-1β secretion in LPS-induced macrophage 264.7 through inhibiting the expression of TREM-1 gene.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Pharmaceutical Journal Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Chinese Pharmaceutical Journal Año: 2013 Tipo del documento: Artículo