Protective effects of Lycium ruthenicum anthocyanins on the vascular endothelial cells with oxidative injury by oxidized low-density lipoprotein in vitro / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the protective effects of Lycium ruthenicum anthocyanins on the oxidative injury of human umbilical vein endothelial cells (HUVEC) caused by in vitro oxidation of low density lipoprotein(ox-LDL). METHODS: The oxidative damage model was established by culturing human umbilical vein endothelial cells with ox-LDL in vitro. The cells were divided into 6 groups; the normal group, ox-LDL group, simvastatin group, low dose of anthocyanin+ox-LDL group, middle dose of anthocyanin+ox-LDL group and high dose of anthocyanin+ox-LDL group. After experimental treatment, cell count and morphologic observation for each dose group. MTT assay was used to detect cell proliferation capacity, flow cytometry (FCM) was used to detected endothelial cell proliferation cycle and apoplosis rate, and the activity of the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and peroxidase (POD), as well as the content of the malondialdehyde (MDA) were detected. RESULTS: Mid and high concentration of Lycium ruthenicum anthocyanins can effectively inhibit vascular endothelial cell injury induced by ox-LDL, slow down the G0/G1, SPR and G2/M ratio and apoptopsis rate of the damaged cells; at the same time, it can also improve the activity of SOD, POD and GSH-Px in cell metabolism, and reduce the content of MDA. CONCLUSION: Lycium ruthenicum anthocyanins play a protective role on humanumbilical vein endothelial cells damaged by ox-LDL, and the mechanism may be related to its antioxidant effect.