HPLC determination of CPT-11 and its metabolite 7-ethyl-10 HCPT in incubation system of rat liver microsomes / 中国药学杂志
Chinese Pharmaceutical Journal
;
(24): 485-488, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-860453
ABSTRACT
OBJECTIVE:
To establish an HPLC method for determing irinotecan (CPT-11) and its metabolite 7-ethyl-10 HCPT in rat liver microsome incubation system, and to optimize the incubation conditions.METHODS:
CPT-11and SN-38 were determined by HPLC. Single factor design was used to optimize the incubation conditions.RESULTS:
The linear range of CPT-11 and 7-ethyl-10 HCPT in rat liver microsome incubation system were 20-4000 ng · mL-1 and 2-400 ng · mL-1, respectively. The optimal incubation conditions were as follows 10 μmol · L-1 CPT-11, 0.02 mg liver microsomes and incubation for 15 min.CONCLUSION:
The HPLC method is accurate and suitable for the determination of CPT-11and 7-ethyl-10 HCPT in rat liver microsomes. The incubation condition can be applied in drug interaction studies of irinotecan.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Pharmaceutical Journal
Año:
2013
Tipo del documento:
Artículo
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