Preparation and in vitro release of lyophilized powder of sorafenib solid lipid nanoparticles / 中国药学杂志

ABSTRACT

OBJECTIVE: To prepare soraienib solid lipid nanoparticles and investigate its physicochemical properties and in vitro release profile. METHODS: Sorafenib solid lipid nanoparticles were prepared by emulsion evaporation-solidification at low temperature. The morphology was examined by transmission electron microscope. The particle size and Zeta potential were determined by laser granularity equipment. The encapsulation efficiency was detected by Sephadex gel chromatography and HPLC. The in vitro release pro-file of sorafenib solid lipid nanoparticles was studied using dialysis technology. The lyophilized powder of sorafenib solid lipid nanoparticles was prepared by refrigerated air dryer and its material phase was analyzed by DSC. RESULTS: The sorafenib solid lipid nanoparticles assumed spherical shape. The distribution of diameter was even with average particle size of (108.2±7.0) nm. The PDI and Zeta potential were (0.250±0.022) and (-16.4±0.7) mV, respectively. The average encapsulation efficiency was (73.49±1.87)%. The release in vitro accorded with Weibull order model. Equal volume of 15% mannitol was the best protective agent for lyophilized powder of sorafenib solid lipid nanoparticles. The formation of a new material phase was testified by analysis of DSC. CONCLUSION: The method of emulsion evaporation-solidification at low temperature was appropriate for preparation of sorafenib solid lipid nanoparticles. The nanoparticles had stable physical properties and significant sustained-release effect.