Simultaneous determination of five index components in Glycyrrhizae Radix et Rhizoma and Longchai Decoction by HPLC / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a determination method of five index components to control the quality of Glycyrrhizae Radix et Rhizoma and Longchai Decoction. METHODS: An ODS C18 column (4.6 mm × 250 mm, 5 μm) was adopted; the mobile phase consisted of acetonitrile -0.05% phosphoric acid with gradient elution at the flow rate of 1 mL · min-1; the detection wavelength was 237 nm, and the column temperature was 30°C. RESULTS: The five index components achieved baseline separation, and the negative sample showed no interference. The linear ranges were 0.156 8-1.568 μg for liquiritin (r=0.999 7), 0.157 1-1.571 μg for isoliquiritin (r=0.999 8), 0.155 8-1.558 μg for liquiritigenin (r=0.999 6), 0.187 1-1.871 μg for glycyrrhizic (r=0.996 9) and 0.158 1-1.581 μg for isoliquiritigenin (r=0.999 5), respectively. The average recoveries were 101.47% (RSD=1.272%), 101.09% (RSD=1.937%), 101.14% (RSD=2.388%), 100.38% (RSD=1.448%) and 100.86% (RSD=1.759%), respectively (n=5). CONCLUSION: This method has good resolution and high precision, and can be used for the quality control of Glycyrrhizae Radix et Rhizoma and Longchai decoction.