UPLC determination of six active components including mangiferin in Anemarrhenae Rhizoma / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish an analytical method to determine 7-O-glucopyranosylmangiferin, mangiferin, timosapon-inB II, 2, 6, 4'-trihydroxy-4-methoxybenzophenone, broussonin B and cis-hinokiresinol in Anemarrhenae Rhizoma quickly and accurately. METHODS: A UPLC method was established on an HSS T3 column (2.1 mm × 100 mm, 1.8 μm). The mobile phase consisted of 0.03% phosphoric acid solution and acetonitrile eluted in gradient mode, and the detection wavelength was set at 192 and 210 nm. RESULTS: The standard curves of 7-O-glucopyranosylmangiferin, mangiferin, timosaponin B II, 2, 6, 4'-trihydroxy-4-methoxy-benzophenone, broussonin B and cis-hinokiresinol showed good linearity in 15.00-299.6 μg · mL-1, 8.80- 175.6 μg · mL-1, 40.00-800.0 μg · mL-1, 2.300- 45.60 μg · mL-1, 4.200-8.50 μg · mL-1 and 0.400-9.00 μg · mL-1, respectively. The mean recoveries (n=9) of the 6 components were 97.7%-100.6% with RSD of 1.2%. CONCLUSION: The method is quick and accurate and can be used to determine 7-O-glucopyranosylmangiferin, mangiferin, timosaponin B II, 2, 6, 4'-trihydroxy-4-methoxy-benzophenone, broussonin B and cis-hinokiresinol in Anemarrhenae Rhizoma.