Expressions and Significance of LncRNA-BBOX1-2 and FGFR1 in Gastric Cancer / 胃肠病学

ABSTRACT

Background: Dysregulation of long non-coding RNAs (lncRNAs) has been found in a variety of malignancies. Previous study revealed that lncRNA-BBOX1-2 was up-regulated in gastric cancer and fibroblast growth factor receptor 1 (FGFR1) might be the potential target gene of lncRNA-BBOX1-2. Aims: To investigate the expressions and significance of lncRNA-BBOX1-2 and FGFR1 in gastric cancer. Methods: Expressions of lncRNA-BBOX1-2 and FGFR1 were detected by real-time PCR in 45 cases of gastric cancer tissues and the adjacent normal tissues. The correlations of these expressions with clinicopathological features of gastric cancer were analyzed. ROC curve analysis was used to assess the performance of lncRNA-BBOX1-2 and FGFR1 in distinguishing cancerous tissue and normal tissue and predicting lymph node metastasis. After transfected with siRNA-BBOX1-2, the expression of FGFR1 in human gastric cancer cell line SGC-7901 was detected by real-time PCR and Western blotting. Results: Both lncRNA-BBOX1-2 and FGFR1 were overexpressed in gastric cancer tissues than in adjacent normal tissues (P<0.05). The expression level of lncRNA-BBOX1-2 was positively correlated with lymph node metastasis and TNM stage (P<0.05), and the expression level of FGFR1 was positively correlated with tumor differentiation, depth of invasion, lymph node metastasis and TNM stage (P<0.05). The expression levels of lncRNA-BBOX1-2 and FGFR1 demonstrated a modest correlation with each other (r=0.344, P<0.05). When lncRNA-BBOX1-2 was knocked out by RNA interference, mRNA and protein expressions of FGFR1 were significantly decreased in SGC-7901 cells (P<0.05). ROC curve analysis showed that lncRNA-BBOX1-2 and FGFR1 might be potential biomarkers for initiation (AUC 0.916 and 0.862, respectively) and lymph node metastasis (AUC 0.720 and 0.774, respectively) of gastric cancer. Conclusions: LncRNA-BBOX1-2 and FGFR1 are up-regulated in gastric cancer tissues. LncRNA-BBOX1-2 might be a positive regulator of FGFR1 and their cooperation are involved in modulating tumorigenesis and development of gastric cancer.