Combination of Blue Laser Exposure with UV-LED to Improve Antimicrobial Effects on Staphylococcus aureus Biofilm

ABSTRACT

@#Introduction: Staphylococcus aureus biofilm is a major mediator of infection. The light based therapy is still not adequate to eradicate biofilm caused by Extracellular Polymeric Substances (EPS). The Ultra Violet (UV) irradiation has high energy and sufficient to penetrate a tissue. This study aims to investigate the effect of combination of UV LED irradiation and blue laser on the biofilm. Methods: The pure culture of Staphylococcus aureus ATCC 25923 approximated 108 CFU/mL or 1.0 McFarland Standard was used for this study. The biofilm sample was placed onto micro plate for 48 hours. The treatment group was divided into 3 groups, which were blue laser group, UV LED group and UV-Blue laser group. Results: The results showed that the highest biofilm reduction (80.57 ± 0.77) % was treated by blue laser irradiation for 4 minutes and UV irradiation for 20 s. Conclusion: Thus, the combination UV LED and Blue laser is the best choice to eradicate more biofilm.