In vitro development of canine somatic cell nuclear transfer embryos in different culture media
Journal of Veterinary Science
;
: 233-235, 2015.
Artículo
en Inglés
| WPRIM
| ID: wpr-86394
ABSTRACT
The objective of the present study was to investigate the effects of three different culture media on the development of canine somatic cell nuclear transfer (SCNT) embryos. Canine cloned embryos were cultured in modified synthetic oviductal fluid (mSOF), porcine zygote medium-3 (PZM-3), or G1/G2 sequential media. Our results showed that the G1/G2 media yielded significantly higher morula and blastocyst development in canine SCNT embryos (26.1% and 7.8%, respectively) compared to PZM-3 (8.5% and 0%) or mSOF (2.3% and 0%) media. In conclusion, this study suggests that blastocysts can be produced more efficiently using G1/G2 media to culture canine SCNT embryos.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Blastocisto
/
Clonación de Organismos
/
Medios de Cultivo
/
Técnicas de Cultivo de Embriones
/
Desarrollo Embrionario
/
Perros
/
Técnicas de Transferencia Nuclear
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2015
Tipo del documento:
Artículo
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