The role of NLRP12 in diabetic patients complicated with coronary heart disease / 中华内分泌外科杂志

ABSTRACT

Objective:To investigate the role of nucleotide-binding oligomerization domain-like receptor protein 12 (NLRP12) in diabetic patients with coronary heart disease.Methods:From Jun. 2017 to Oct. 2018, 40 patients with type 2 diabetes mellitus (T2DM) in our hospital were selected as simple diabetes group, 80 patients with coronary heart disease as simple coronary heart disease group, 80 patients with T2DM and coronary heart disease as diabetes mellitus complicated with coronary heart disease group, and 60 healthy people in the same period as control group. Peripheral blood mononuclear cells were isolated and cultured. The levels of NLRP12, apoptosis-related spot-like protein, Caspase-3 mRNA and protein expression were detected by RT-PCR and Western blot. The plasma was separated and the content of IL-18 was detected by ELISA.Results:The relative expressions of NLRP12, apoptosis-related spot-like protein, Caspase-3 mRNA and protein in peripheral blood mononuclear cells of diabetes mellitus group, coronary heart disease group and diabetes mellitus complicated with coronary heart disease group were significantly higher than those of the control group ( P<0.05) . The apoptosis-related spot-like protein and Caspase-3 mRNA in peripheral blood mononuclear cells of diabetes mellitus complicated with coronary heart disease group were significantly higher than those of the control group ( P<0.05) . The relative expression of NLRP12 was significantly higher than that of diabetes mellitus group and coronary heart disease group ( P<0.05) . There was no significant difference in the relative expression of NLRP12 gene and protein between diabetes mellitus group, coronary heart disease group and diabetes mellitus complicated with coronary heart disease group ( P>0.05) . The plasma levels of IL-18 in diabetes mellitus group, coronary heart disease group and diabetes mellitus complicated with coronary heart disease group were significantly higher than those in the control group ( P<0.01) ; the plasma levels of IL-18 in diabetes complicated with coronary heart disease group were significantly higher than those in diabetes mellitus group and coronary heart disease group ( P<0.05) . The correlation analysis showed that plasma IL-18 level was positively correlated with NLRP12, apoptosis-related spot-like protein, Caspase-3 mRNA and relative protein expression. Conclusion:The abnormal activation of NLRP12 promotes the maturation and release of downstream inflammatory factor IL-18 by activating the expression of apoptosis related spot like protein and Caspase-3, and participates in the occurrence and development of diabetes mellitus complicated with coronary heart disease.