Adiponectin Upregulates Filaggrin Expression via SIRT1-Mediated Signaling in Human Normal Keratinocytes
Annals of Dermatology
;
: 407-413, 2017.
Artículo
en Inglés
| WPRIM
| ID: wpr-86521
ABSTRACT
BACKGROUND:
Filaggrin (FLG) is the major component of the epidermal granular layer and binds to and condenses the keratin cytoskeleton. FLG thus contributes to cell compaction and serves as a natural moisturizing factor by promoting unfolding and degradation into hygroscopic amino acids. Loss or downregulation of FLG has been shown to result in a weak stratum corneum, which causes water loss and increases the possibility of skin barrier-related seizure. Adiponectin (Acrp30) contributes to the functional recovery of somatic cells, including human normal epidermal keratinocytes (NHEKs).OBJECTIVE:
To investigate the effect of Acrp30 in FLG expression and identifying its signal transduction mechanism.METHODS:
Normal human keratinocytes were treated with Acrp30 and the levels of FLG were examined. Silent mating type information regulation 2 homolog (SIRT)-targeting siRNA and aryl hydrocarbon receptor nuclear translocator (ARNT)-targeting siRNA were used to identify the role of various signal transduction pathway components.RESULTS:
Acrp30 upregulated SIRT1 and ARNT expression in NHEKs, resulting in increased FLG expression. Treatment with both SIRT1-targeting siRNA and ARNT-targeting siRNA blocked Acrp30 stimulation and silenced FLG expression.CONCLUSION:
Adiponectin upregulates FLG expression through a SIRT1-mediated pathway. Our results suggest that Acrp30 is a promising agent for skin barrier permeability improvement.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Permeabilidad
/
Convulsiones
/
Piel
/
Citoesqueleto
/
Agua
/
Transducción de Señal
/
Regulación hacia Abajo
/
Queratinocitos
/
ARN Interferente Pequeño
/
Adiponectina
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Inglés
Revista:
Annals of Dermatology
Año:
2017
Tipo del documento:
Artículo
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