Effect of preoperative sleep deprivation on intestinal microflora of immunosuppressed mice after operation / 中华麻醉学杂志

ABSTRACT

Objective:To evaluate the effect of preoperative sleep deprivation on the intestinal microflora of immunosuppressed mice after operation.Methods:Seventy-two SPF healthy adult male C57BL/6J mice, aged 8-10 weeks, weighing 23-25 g, were divided into 5 groups using a random number table method: operation group (group O, n=18), preoperative sleep deprivation group (group SR, n=18), pseudo germ-free mice orally treated with normal saline group (group GF+ V, n=12), pseudo germ-free mice that received fecal bacteria transplants from mice in group O group (group GF+ C, n=12), and pseudo germ-free mice that received fecal bacteria transplants from mice in group SR group (group GF+ SR, n=12). In group O and group SR, mice were subjected to sleep deprivation for 20 h every day for 7 consecutive days starting from 7 days before intraperitoneal exploration and partial liver lobectomy.Broad-spectrum antibiotics dissolved in drinking water were given ad libitum to C57BL/6 mice for 14 consecutive days starting from 2 weeks before operation to establish the pseudo germ-free mice model.Pseudo germ-free mice were gavaged with normal saline or fecal suspension (200 μl) obtained from mice at 24 h after surgery in group O and group SR.The fecal samples were collected at 24 h after operation for 16S rRNA gene sequence analysis.The longitudinal diameter and weight of the spleen were measured at 24 h after operation or fecal microbiota transplantation (FMT). CD4 + T cells, CD8 + T cells and regulatory T cells (Treg cells) were detected by flow cytometry. Results:Compared with group O, the Simpson diversity index of intestinal microflora was significantly decreased, the number of CD4+ T cells and CD8+ T cells in blood was decreased, the percentage of Treg cells was increased, and the longitudinal diameter and weight of the spleen were decreased in group SR, and the numbers of CD4 + and CD8 + cells in blood were significantly decreased, the percentage of Treg cells in blood was increased, and the longitudinal diameter and weight of the spleen were decreased in group GF+ V ( P<0.05). Compared with group GF+ V, the number of CD4 + T cells and CD8 + T cells in the blood was significantly increased, the percentage of Treg cells was decreased, and the longitudinal diameter and weight of the spleen were increased in group GF+ O ( P<0.05). Compared with group GF+ O, the number of CD4+ T cells and CD8+ T cells in blood was significantly decreased, the percentage of Treg cells was increased, and the longitudinal diameter and weight of the spleen were decreased in group GF+ SR ( P<0.05). Conclusion:The mechanism by which preoperative sleep deprivation accentuates postoperative immunosuppression is related to the intestinal microflora disorder in mice.