Cisplatin induces senescence of hepatoma cells via p19/p21 pathway / 中华普通外科杂志

ABSTRACT

Objective:To investigate the induction mechanism of cisplatin on hepatoma senescence.Methods:2 μg/ml cisplatin was applied to affect hepatocellular carcinoma cell line HepG2. Cell proliferation of tumor cells was detected by MTS method. The cell cycle was detected by flow cytometry. The RT-PCR and Western blot were used to detect the expression of senescence regulation genes, p19 and p21. The expression of p21 was detected by cell immunofluorescence. Nude mouse model of human hepatocellular carcinoma was intraperitoneally injected with specific drug to measure the growth, metastasis of tomor and expression of p21 protein.Results:Cell proliferation rate in cisplatin group was lower than that of the control group( t=8.958, P<0.05). Flow cytometry showed the percentage of G 2 phase were19.90%±0.42%, 12.57%±0.84% in the cisplatin and control group, respectively. Cells were arrested in G 2 phase ( t=14.415, P<0.05). In the cisplatin group and control group, the relative expression of p19 were(2.23±0.05), (1.00±0.02); the relative expression of p21 were (3.26±0.11), (1.00±0.02). The expression of p19 and p21 increased(respectively t=43.750, 56.541, all P<0.05). The tumor size of cisplatin group was smaller than that of the control group, and the expression level of p21 protein was higher than that of the control group ( P<0.05). Conclusion:Cisplatin can induce senescence of hepatocellular carcinoma cells through the p19/p21 pathway.