Prevalence and characteristics of clinical Aeromonas isolates carrying mcr-3 gene / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
;
(12): 697-702, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-871339
ABSTRACT
Objective:
To investigate the prevalence and characteristics of mcr genes in clinical isolates of Aeromonas spp. in our hospital, and provide reference for clinical analysis of the prevalence and expression of colistin resistance genes.Methods:
Polymerase chain reaction (PCR) was used to detect mcr genes in 183 Aeromonas spp. strains. The minimum inhibitory concentrations (MICs) of colistin and polymyxin against mcr-positive Aeromonas spp. were detected by micro broth dilution method. Broth conjugation and filter mating conjugation were performed. Whole genome sequencing was used to analyze the genetic environment of mcr-3 gene in Aeromonas spp.. A recombinant Escherichia coli ( E. coli) DH5α-pGEM-T p mcr-3 strain was constructed to verify the expression of mcr-3 gene.Results:
The positive rate of mcr-3 gene in 183 strains of Aeromonas spp. was 2.19% (4/183). No mcr-1 or mcr-2 gene was detected among these isolates. Antimicrobial susceptibility test showed that four mcr-3-carrying Aeromonas hydrophilia ( A. hydrophilia) strains were sensitive to colistin and polymyxin (MIC<2 μg/ml). Conjugation experiments indicated that mcr-3 gene could not be transferred between strains. Whole-genome sequencing analysis suggested that the mcr-3 genes carried by the A. hydrophilia isolates belonged to mcr-3.2 and mcr-3-like variants, and no adjacent transfer element was detected upstream and downstream. The recombinant E. coli DH5α-pGEM-T p mcr-3 strain was sensitive to colistin (MIC=2 μg/ml).Conclusions:
The clinical isolates of A. hydrophilia in our hospital carried mcr-3 gene, but does not exhibit colistin resistance, and no evidence supported the transfer of mcr-3 gene for the time being.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio de prevalencia
Idioma:
Chino
Revista:
Chinese Journal of Microbiology and Immunology
Año:
2020
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS