Effects of Toluene on the Development of the Inner Ear and Lateral Line Sensory System of Zebrafish / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 110-118, 2021.
Artículo
en Inglés
| WPRIM
| ID: wpr-878327
ABSTRACT
Objective@#The aim of this study was to explore the ototoxicity of toluene in the early development of zebrafish embryos/larvae.@*Methods@#Zebrafish were utilized to explore the ototoxicity of toluene. Locomotion analysis, immunofluorescence, and qPCR were used to understand the phenotypes and molecular mechanisms of toluene ototoxicity.@*Results@#The results demonstrated that at 2 mmol/L, toluene induced zebrafish larvae death at 120 hours post fertilization (hpf) at a rate of 25.79% and inhibited the rate of hatching at 72 hpf. Furthermore, toluene exposure inhibited the distance travelled and average swimming velocity of zebrafish larvae while increasing the frequency of movements. As shown by fluorescence staining of hair cells, toluene inhibited the formation of lateral line neuromasts and middle line 1 (Ml @*Conclusion@#This study indicated that toluene may affect the development of both the inner ear and lateral line systems in zebrafish, while the lateral line system may be more sensitive to toluene than the inner ear.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Tolueno
/
Pez Cebra
/
Regulación del Desarrollo de la Expresión Génica
/
Embrión no Mamífero
/
Sistema de la Línea Lateral
/
Ototoxicidad
/
Células Ciliadas Auditivas
/
Oído Interno
/
Locomoción
Límite:
Animales
Idioma:
Inglés
Revista:
Biomedical and Environmental Sciences
Año:
2021
Tipo del documento:
Artículo
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