Construction of CAR-T cells targeting CS1 and analysis of their antitumor activity in vitro / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2162-2170, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-878475
ABSTRACT
We constructed the CS1-targeted second- and third-generation CAR-T cells with genetic engineered 4-1BB or/and ICOS as a costimulatory signaling molecule by use of lentiviral platform. The CS1-targeted second-generation CAR-T cells with ICOS or 4-1BB had similar anti-neoplastic activity. When effector/target ratio was 11, the CAR-T cells with ICOS showed better killing effect on IM9-lucgfp cells than those with 4-1BB. However, The CS1-targeted third-generation CAR-T cells exihibited lower cytolytic capacity against IM9-lucgfp cells than the CS1-targeted second-generation CAR-T cells when the ratio of effector/target was 11, 21 or 51. When the ratio of effector/target was 101, the killing efficacy of both the second- and third-generation CAR-T cells against IM9-lucgfp cells was more than 85%, significantly higher than that of the control T cells. Taken together, both the CS1-targeted second- and third-generation CAR-T cells with ICOS or/and 4-1BB could efficiently kill CS1-positive multiple myeloma cells, but the CS1-targeted second-generation CAR-T cells had more potent killing effect on CS1-positive multiple myeloma cells than the CS1-targeted third-generation CAR-T cells.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Linfocitos T
/
Transducción de Señal
/
Ingeniería Genética
/
Ensayos Antitumor por Modelo de Xenoinjerto
/
Línea Celular Tumoral
/
Ligando 4-1BB
/
Proteína Coestimuladora de Linfocitos T Inducibles
/
Mieloma Múltiple
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2020
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS