Progress in the construction and screening of random mutation library / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 163-177, 2021.
Artículo
en Chino
| WPRIM
| ID: wpr-878551
ABSTRACT
Directed evolution is a cyclic process that alternates between constructing different genes and screening functional gene variants. It has been widely used in optimization and analysis of DNA sequence, gene function and protein structure. It includes random gene libraries construction, gene expression in suitable hosts and mutant libraries screening. The key to construct gene library is the storage capacity and mutation diversity, to screen is high sensitivity and high throughput. This review discusses the latest advances in directed evolution. These new technologies greatly accelerate and simplify the traditional directional evolution process and promote the development of directed evolution.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Secuencia de Bases
/
Proteínas
/
Biblioteca de Genes
/
Evolución Molecular Dirigida
/
Mutación
Tipo de estudio:
Ensayo Clínico Controlado
/
Estudio diagnóstico
/
Estudio de tamizaje
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2021
Tipo del documento:
Artículo
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