Hsa-let-7b-5p Inhibits Proliferation of Human Leukemia THP-1 Cells via FTO/m / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1873-1879, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-879986
ABSTRACT
OBJECTIVE@#To investigate the down-regulation effect of let-7b-5p on the expression of FTO in acute myeloid leukemia cell line THP-1 and inhibitory effect on THP-1 proliferation via m@*METHODS@#The acute myeloid leukemia cell line THP-1 and the normal human peripheral blood mononuclear cells (PBMNC) were selected as subjects. The expression of let-7b-5p and FTO mRNA in those cells was detected by qPCR, further the expression of FTO protein in those cells was detected by Western blot. And, the luciferase reporter gene assay was used to verify the targeting effect of let-7b-5p on FTO. Finally, THP-1 cells were transfected respectively with let-7b-5p mimic, and PBMNC with let-7b-5p inhibitor, there after the C-MYC mRNA m@*RESULTS@#Compared with PBMNC, the expression of let-7b-5p in THP-1 significantly decreased, while the expression of FTO was significantly increased (P<0.05). After transfection with let-7b-5p mimic combined with FTO 3'-UTR, the luciferase activity of transfected THP-1 cells significantly decreased, but the luciferase activity significantly increased after transfection with mutant 3'-UTR, which was significantly different from the negative control group(blank vector) (P<0.05). Let-7b-5p inhibitor down-regulated c-MYC mRNA m@*CONCLUSION@#Human acute myeloid leukemia cell line THP-1 low expresses the let-7b-5p, which regulates c-MYC expression through let-7b-5p-/FTO-/m
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Leucocitos Mononucleares
/
Transducción de Señal
/
Leucemia
/
MicroARNs
/
Línea Celular Tumoral
/
Proliferación Celular
/
Dioxigenasa FTO Dependiente de Alfa-Cetoglutarato
/
Células THP-1
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2020
Tipo del documento:
Artículo
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