Mechanisms for propofol in inhibiting the proliferation and invasion of glioma U87 cells and its effect on miR-134 expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 18-24, 2021.
Artículo
en Inglés
| WPRIM
| ID: wpr-880617
ABSTRACT
OBJECTIVES@#To investigate the effects of propofol on the proliferation and invasion of glioma U87 cells and to explore the possible anti-tumor mechanisms.@*METHODS@#The glioma U87 cells was divided into a blank group, a positive control group, and the propofol groups (1.00, 2.00 or 5.00 mmol/L). Cell counting kit-8 (CCK-8) was used to detect cell proliferation; Transwell method was used to detect the effect of propofol on invasion and migration of U87 cells; real-time PCR was used to detect the expression of microRNA-134 (miR-134); Western blotting was used to detect the expression levels of reproduction-related protein Ki-67, invasion-related protein metalloproteinase-2 (MMP-2), metalloproteinase-9 (MMP-9) and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) signaling pathway-related protein.@*RESULTS@#Compared with the blank group, the proliferation, invasion and migration capacity of U87 cells were reduced in the positive control group and the propofol groups after 48 hours (all @*CONCLUSIONS@#Propofol can decrease the proliferation rate, and the invasion and migration abilities of U87 cells, which may be achieved by up-regulation of miR-134 and suppression of PI3K/Akt signaling pathway.
Texto completo:
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Propofol
/
Movimiento Celular
/
Fosfatidilinositol 3-Quinasas
/
Metaloproteinasa 2 de la Matriz
/
MicroARNs
/
Línea Celular Tumoral
/
Proliferación Celular
/
Proteínas Proto-Oncogénicas c-akt
/
Glioma
Límite:
Humanos
Idioma:
Inglés
Revista:
Journal of Central South University(Medical Sciences)
Año:
2021
Tipo del documento:
Artículo
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