Damaging effect of nano-neodymium oxide on PC12 cells / 中国职业医学

ABSTRACT

OBJECTIVE: To study the damaging effect of nano-neodymium oxide( Nano-Nd_2O_3) on rat adrenal pheochromocytoma cell PC12. METHODS: PC12 cells in the logarithmic growth phase were divided into control group and 7 drug treatment groups. The control group was not treated with Nano-Nd_2O_3. The drug treatment groups were treated with 7 different dosing with the final mass concentrations of 3. 13,6. 25,12. 50,25. 00,50. 00,75. 00,100. 00 mg/L of NanoNd_2O_3 for 24 hours,then the follow-up selected experimental concentration were 3. 13,6. 25,12. 50,25. 00,50. 00 and75. 00 mg/L. The CCK-8 essay was used to detect the cell viability. The levels of glutathione( GSH) and superoxide dismutase( SOD) in the cells were measured by enzyme-linked immunosorbent assay. The expression of B cell lymphoma-2( Bcl-2),Bcl-2 associated X protein( Bax) and Caspase-3 protein were detected by Western blot. RESULTS: The survival rate,GSH level and Bcl-2/Bax ratio of PC12 cells decreased with the increase of the Nano-Nd_2O_3 dose,and showed doseresponse relationship( P < 0. 01). The SOD activity of PC12 cells in Nano-Nd_2O_3 group of 12. 50,25. 00,50. 00 and75. 00 mg/L were lower than that of control group( P < 0. 05). The ratio of Caspase-3/β-actin in PC12 cells was higher than that in control group( P < 0. 05). In the range of 0. 00-25. 00 mg/L of Nano-Nd_2O_3,with the increase of NanoNd_2O_3 dose,the ratio of Caspase-3/β-actin in PC12 cells increased gradually,and showed a dose-response relationship( P < 0. 01). CONCLUSION: Nano-Nd_2O_3 can induce oxidative stress in PC12 cells,leading to the decrease of Bcl-2/Bax ratio,activate Caspase-3 expression,and than inhibits cell proliferation in a dose-dependent manner.