Effect of salidroside on autophagy of pancreatic cells in rats with severe acute pancreatitis / 中华急诊医学杂志

ABSTRACT

Objective:To study the effects of salidroside (Sal) on autophagy of pancreatic cells in rats with severe acute pancreatitis (SAP).Methods:Fifty healthy male Wistar rats were randomly (random number) divided into the sham group, SAP group, Sal 15 group, Sal 30 group and Sal 60 group, 10 rats in each group. The sham group was injected retrogradely normal saline into pancreatic duct, while the 5% sodium taurocholate was injected retrogradely into pancreatic duct to establish severe pancreatitis model in the SAP group and all the Sal groups. Salidroside at different doses were intraperitoneally injected in all the Sal groups at 2 h after surgery. The serum amylase (AMY) activity and plasma tumour necrosis factor-α (TNF-α) level were detected by enzyme-linked immunosorbent assay (ELISA), and Western blot was used to detect the expression of pancreatic tissue protein. The histopathological changes of pancreas were observed under the light microscope. The data were analyzed by SPSS 24.0, and One-way ANOVA was used for comparison between groups. A P value less than 0.05 was considered to be statistically significant. Results:After salidroside intervention, the levels of TNF-α (28.1±0.9) pg/mL, the relative expression of p65 (0.52±0.01), AMY activity (3 801±292) and relative expression of p-mTOR were the lowest in the Sal 60 group (all P<0.05); while the relative expression of LC3 was the highest in the Sal 60 group (all P <0.05). The degree of pathological damage of pancreatic tissues was decreased with the increase of salidroside dose. Conclusions:Salidroside at different doses have protective effects on rats with severe pancreatitis, which seems to be most obvious in the Sal 60 group.