Endothelial F-actin changes in the alkali burned rabbit cornea
Yonsei Medical Journal
;
: 484-492, 1994.
Artículo
en Inglés
| WPRIM
| ID: wpr-88509
ABSTRACT
The healing mechanism of corneal endothelium after alkali burn was not completely understood. Rabbit cornea was burned with 1N sodium hydxoside for 1 minute. Endothelial F-actin was stained with NBD-phallacidin in regular sequence to find out the details of endothelial healing after alkali burn. F-actin was completely destroyed leaving a sharp margin against the unaffected area 1 hour after the burn. In the 3, 5 and 7 day specimens, highly active F-actin reactions were noted at the wound margin. New multiple F-actin layers, arising from the intact endothelium near the wound margin, were noted in the 9 day specimen. In the 8 1/2 month specimen, the endothelial defected area was covered by large primitive cells, each of which showed F-actin fiber bundles in the cytoplasm with a large nuclear shadow. Nearly all of the large primitive cells showed F-actin fibers arranged in shapes of cell junctions. Twelve months after the burn, endothelial defects were not found. Nearly all of the endothelial cells were normal in size and shape except for some mushroom-like projections toward the anterior chamber in some areas. Nineteen months after the burn, the endothelial cells were normal. Endothelial wound healing process can be continued even 1 year after the alkali burn in rabbit cornea.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Hidróxido de Sodio
/
Cicatrización de Heridas
/
Quemaduras Químicas
/
Endotelio Corneal
/
Quemaduras Oculares
/
Actinas
/
Animales
Límite:
Animales
Idioma:
Inglés
Revista:
Yonsei Medical Journal
Año:
1994
Tipo del documento:
Artículo
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