Generation and phenotypic characterization of S100A9 gene knockout mice by CRISPR/Cas9-mediated gene targeting / 生理学报
Acta Physiologica Sinica
;
(6): 482-490, 2021.
Artículo
en Chino
| WPRIM
| ID: wpr-887683
ABSTRACT
S100 calcium binding protein A9 (S100A9) is involved in a variety of biological processes such as inflammation and tumor cell migration and invasion regulation. The purpose of this study was to construct S100A9 gene-edited mice by using CRISPR/Cas9 technology, thereby providing an animal model for exploring the biological functions of this gene. According to the S100A9 gene sequence, the single-stranded small guide RNA (sgRNA) targeting exons 2 and 3 was transcribed in vitro, and a mixture of Cas9 mRNA and candidate sgRNA was injected into mouse fertilized eggs by microinjection. Early embryos were obtained and transferred to surrogate mice, and F
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fenotipo
/
Líquido del Lavado Bronquioalveolar
/
Ovalbúmina
/
Ratones Noqueados
/
Marcación de Gen
/
Calgranulina B
/
Modelos Animales de Enfermedad
/
Técnicas de Inactivación de Genes
/
Sistemas CRISPR-Cas
/
Pulmón
Límite:
Animales
Idioma:
Chino
Revista:
Acta Physiologica Sinica
Año:
2021
Tipo del documento:
Artículo
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