Research progress in the rapid preparation of monoclonal antibodies of mouse hybridoma / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 2293-2306, 2021.
Artículo
en Chino
| WPRIM
| ID: wpr-887797
ABSTRACT
Mouse hybridoma monoclonal antibody is the most commonly used antibody in immunology because of its stable source, easy preparation in later stage and high yield. The traditional time-consuming and laborious hybridoma preparation technology could not meet the growing market demand. In this paper, we describe the rapid preparation techniques involved in antigen design and screening, B cell enrichment and screening, transgenic myeloma cells, fusion technology improvement, positive hybridoma cell screening and rapid detection of monoclonal antibody performance, to provide a reference for rapid preparation of mouse hybridoma monoclonal antibody.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Linfocitos B
/
Hibridomas
/
Anticuerpos Monoclonales
/
Antígenos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Biotechnology
Año:
2021
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS