Rapid chemome profiling of Cistanche salsa using DI-MS/MS~(ALL) / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 4150-4156, 2021.
Artículo
en Chino
| WPRIM
| ID: wpr-888075
ABSTRACT
The current study aims to rapidly and comprehensively profile the chemical composition of Cistanche salsa using direct infusion coupled with MS/MS~(ALL)(DI-MS/MS~(ALL)). The C. salsa extract was directly imported into electrospray ionization(ESI) source of quadrupole time-of-flight(Q-TOF) mass spectrometer with an infusion pump at a flow rate of 10 μL·min~(-1). Acquisition program was applied under negative ionization polarity to collect one MS~1 spectrum(m/z 50-1 200), followed by 1 150 MS~2 spectra with precursor isolation window(m/z 1) amongst mass range m/z 50-1 200. After each MS~2 spectrum was matched to its precursor ion, putative identification was conducted through matching mass spectral data with literature and database. A total of 31 components were identified from C. salsa, including 9 phenylethanoid glycosides, 2 iridoids, 4 saccharides, 9 organic acids, and 7 other compounds, similar to those from C. tubulosa and C. deserticola. In conclusion, DI-MS/MS~(ALL), a facile and reliable analytical tool, can be employed for qualitative analysis of chemical constituents in C. salsa. The research offers a promising strategy to achieve rapid chemome profiling of herbal medicine and provides an alternative source of Cistanches Herba.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Medicamentos Herbarios Chinos
/
Cromatografía Líquida de Alta Presión
/
Cistanche
/
Espectrometría de Masas en Tándem
/
Glicósidos
Tipo de estudio:
Investigación cualitativa
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2021
Tipo del documento:
Artículo
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